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When diminution in pH accompanied the anoxia, oxidative decarboxylation was subsequently decreased to less than 50$ of normal with uniformly labelled glucose as substrate. Uniformly labelled glutamic acid differed from glucose under these circumstances in that the percent of CO2 released from the incubated tissue remained unchanged both following anoxia alone and with accompanying acidosis. A technique for precipitating tissue sodium jLn situ as a relatively insoluble and electron-dense compound (sodium antimonate) was applied to the electron microscopic study of the incubating brain slices.

And (1947) J. , 89, 289. 60 RESPIRATORY CONTROL OF NORMAL AND OF ß-INTERNAL AND γ-EXTERNAL IRRADIATED CNS MITOCHONDRIA Enrique Egana Laboratory of Neurochemistry, Institute of Experimental Medicine, School of Medicine, University of Chile, Santiago, Chile It has been shown that low doses of ß-internal irradiation enhance the fjLn vitro 1 respiration of CNS slices taken from various areas of the neuraxis (from brain cortex to spinal cord). e. the phosphate acceptor ADP and P^, of CNS mitochondria.

Breckenridge and Robert E . Johnston Department of Pharmacology and Beaumont-May Institute of Neurology, Washington University School of Medicine, St. Louis, Missouri. Adenosine 3 ! >5 f -phosphate (3>5-AMP), which mediates certain actions of catecholamines and of 5-hydroxytryptamine, is inactivated by hydrolysis of the 3 ! -P bond. The enzyme responsible, cyclic nucleotide phosphodiesterase, occurs at a high level of activity in the brain (DRUMMOND & PERROTTYEE, 1961). Some concentration of enzyme activity has been noted in synaptosomes (CHEUNG & SALGANICOFF, 1966) and in glial cells ( SHANTA _et al.

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