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5 kDa less t h a n the cellular link protein known to con­ tain one asparagine-linked carbohydrate chain. Consistent with this data, Lohmander et al. (1983) have shown t h a t when addition of this carbohydrate chain is inhibited, the size of link protein synthesized by rat chondrosarcoma chondrocytes is slightly smaller t h a n the secreted link protein. As the size of the translated product is very similar in size to the unglycosylated mature link protein, it seems unlikely t h a t link protein is biosynthesized as a much larger precursor.

P. 5. A c a d e m i c P r e s s , N e w York. , a n d Y a m a g a t a , Τ. (1984). Biochim. Biophys. Acta 7 9 7 , 221-230. , and Hoffman, P. (1965). J. Biol. Chem. 2 4 0 , 1005-1010. , and S u z u k i , S. (1975). J . Biol. Chem. 2 5 0 , 9 1 2 - 9 1 7 . , T a n g , P. , H o u n s e l l , Ε. , and Feizi, Τ. (1986). Eur. J. Biochem. 157, 3 8 5 - 3 9 1 . F u n d e r b u r g h , J. , S t e n z e l - J o h n s o n , P. , a n d Chandler, J. W. (1983). Curr. Eye Res. 2, 7 6 9 - 7 7 6 . , Gregory, J.

1 9 8 6 , for details). T h e e x t e n s i o n of l a n e 8 s h o w s t h a t t h e upper b a n d c a n b e resolved into t w o b a n d s if electrophoresis is prolonged. T h e cloned c D N A hybridized to m R N A s of a p p r o x i m a t e l y 3 . 0 k b . (A) Reproduced from D o e g e et al. (1986b); (Β) reproduced from D e a k et al. (1986). 48 LINDA J. SANDELL seven amino acids present in both bovine and rat chondrosarcoma link proteins. 5 kb of mRNA and includes the entire link protein sequence.

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