By Vladimir Gouli, Svetlana Gouli, Jose Marcelino
This guide supplies a entire and copious illustrated description, with unique artwork, of the commonest ailments in laboratory reared insect colonies, comprising Viruses (Baculoviridae, Reoviridae, Poxviridae, Iridoviridae); micro organism (Bacillaceae, Pseudomonadaceae, Enterobacteriaceae); Rickettsia; Fungi and Microsporidia and Protozoa. Gregarine and Coccidian parasitoids also are depicted. Manuals for the diagnostic of insect illnesses were released within the 90’s, in spite of the fact that, those have been meant for pro insect pathologists or for expert education in invertebrate pathology, for this reason requiring a few clinical historical past in insect pathology. presently, so much courses are net established, now not accomplished and exclusively addressing the commonest illnesses in a selected insect, or workforce of bugs, reared in laboratory amenities. A complete and knowledgeable sensible instruction manual for college students and technicians operating with insect reared colonies is missing within the present literature.
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Additional info for Common Infectious Diseases of Insects in Culture: Diagnostic and Prophylactic Methods
Sporonts are a characteristic feature of microsporidia. For example, species in the genus Nosema produce spores distributed in sporonts; species in the genus Pleistophora present C16 spores per sporont whereas the species in the genus Thelohania present 8 spores per sporont. Microsporidia spores show a complex morphological structure. The spore wall has three layers including external proteinaceous, middle chitinous, and internal plasmatic membrane (Fig. 40). The spore structure is adapted for penetration into host cells through a special morphological structure, the hollow polar tube (polar filament).
Sometimes the simple microscopic analysis using a ‘‘squashing drop method’’ can led to the identification of the insect disease. This method includes the following procedures: 1. Pick up a droplet of water on a slide. 2. Pick up a small amount of insect excrements (closely in size to the water droplet). 3. Mix up the droplet of water with excrement. 4. Cover the mixture with a cover slide and examine under a phase contrast microscope. V. 1007/978-94-007-1890-6_4, Ó Vladimir Gouli 2011 45 46 4 Methods of Practical Diagnostic Fig.
3a, b). Since hemolymph is liquid, slide preparation does not demand complex histological methodology for microscopic analysis. A complete hematological analysis involves the establishment of a ratio between the different types of insect blood cells. However, it is possible to conduct a more simple analysis. Analysis of the fat body is conducted in order to expose viral inclusions such as nuclear polyhedra, granules, poxvirus rhomboidal or ovoid viral formations and different stages of microsporidia.