By Michail V. Sitkovsky, Pierre A. Henkart
Our motivation for placing jointly this publication used to be the necessity for a unmarried resource reference which may be used as an creation to cell-mediated cytotoxicity for beginners to this box, resembling scholars and fellows starting paintings in our laboratories. at this time no such booklet is accessible, and we felt that it'd be necessary as a educating device and as a manner of conveying our enthusiasm approximately contemporary growth within the cytotoxicity box to our colleagues in allied components. It used to be with a few hesitation that we approached our colleagues with the inspiration for this ebook, and we have been happy to discover them very supportive of the belief and prepared to take part. We idea it very important to increase the scope of the booklet to incorporate old, molecular, mobile organic, and medical features of cell-mediated cytotoxicity. To our wisdom this can be the 1st publication on cell-mediated cytotoxicity with one of these wide scope. traditionally, reviews on mobile cytotoxicity have been a part of mobile immunology from its beginning. One improvement of large import used to be the arrival of the fifty one Cr assay, which allowed this arm of the immune reaction to be measured simply and quantitatively. hence, a readout of this effector pathway is on the market inside of a number of hours; different immune effector features can take days or perhaps longer to assay, and the assays are frequently much less quantitative.
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Extra resources for Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods
This has been confirmed (Schick and Berke, 1978), although it appears that blockage of precursor transport can explain the lack of incorporation, and the degree of internal metabolic blockade is unresolved. , 1990). , 1990) show pronounced inhibition oflysis. , 1991), possibly because it interferes directly with the fragmentation mechanism which may involve topoisomerases (Nishioka and Welsh, 1991). , 1990); the inhibition was attributed to the target, but as presented the data do not rule out a reversible action on the CTL.
1989c). Hematopoietic cell lines commonly used in CTL research because of their high sensitivity to lysis show, in general, much more dramatic DNA fragmentation than do fibroblasts or epithelial cells, which are less easily lysed (Howell and Martz, 1987; Sellins and Cohen, 1991). , 1991). As mentioned above, programmed cell death usually required hours for the expression of a genetic program before DNA fragmentation was initiated; inhibition of gene expression delayed or prevented the DNA fragmentation.
These findings strongly support the conclusion that antibody to CD8 has no direct inhibitory effect on programming for lysis, but inhibits indirectly by inhibiting adhesion. Thus they support the conclusion that adhesion is necessary for effective killing. , 1983). , 1982a, 1982b; LFA-I was the first lymphocyte receptor to be discovered in this way). , 1984). , 1990; Moy and Brian, 1992). Antibody-induced inhibition of cytolysis could result from (I) inhibitory signals, (2) interference with generation of stimulatory signals normally resulting from interaction with ICAM-I, or (3) interfer- 17 ence with adhesion per se.