By Paul Singleton(auth.)

DNA know-how is evolving quickly, with new equipment and a fast-growing vocabulary. This detailed dictionary deals present, unique and obtainable info on DNA expertise to academics, researchers and scholars through the biomedical and comparable sciences.

The 3rd variation is an enormous replace, with over 3000 references from mainstream journals and knowledge from the very most modern examine – going way past the remit of such a lot technology dictionaries. It offers transparent motives of phrases, thoughts, and checks, together with advertisement structures, with specific assurance of many vital methods and techniques, and  comprises essay-style entries on many significant themes to help novices to the sector. It covers issues proper to medication (diagnosis, genetic issues, gene therapy); veterinary technology; biotechnology; biochemistry; pharmaceutical science/drug improvement; molecular biology; microbiology; epidemiology; genomics; environmental technological know-how; plant science/agriculture; taxonomy; and forensic science.


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Additional info for Dictionary of DNA and Genome Technology, Third Edition

Example text

This colony can then be used as an inoculum to grow more of the cells which contain the specific cDNA. This procedure clearly depends on efficient and specific protein–antibody binding. A protein synthesized in bacteria from a eukaryotic gene may lack the three-dimensional structure and/or post-translational modification that characterizes the native eukaryotic protein; it may therefore fail to bind antibodies raised against the native protein. ) However, it is possible to produce antibodies that recognise, and bind to, a short, specific run of amino acid residues, even when the target protein lacks the conformation and/or post-translational modification of the native protein.

Caspase activation apparently occurs primarily by cleavage of the zymogen. In at least some cases, one caspase may be activated by another, upstream caspase in a caspase cascade. Fluorophore-labeled probes, which label active caspases in vivo (‘activity-based probes’), allowed researchers to monitor the kinetics of apoptosis in live mice [Nature Med (2009) 15: 967–973]. Apoptosis induced by bacteria In macrophages which have taken up the bacterium Shigella flexneri (by phagocytosis), apoptosis in the macrophage can be promoted by a plasmid-encoded protein, IpaB, secreted by S.

Antibody-based library screening One alternative to screening with a nucleic-acid-based probe (cf. COLONY HYBRIDIZATION). 26 Antibody-based library screening: screening a cDNA expression library (diagrammatic). The cDNA library – consisting of proteinencoding cDNAs within expression vectors – was inserted into a population of bacteria; these bacteria were then inoculated onto agar plates in such a way that each bacterium gave rise to an individual colony. The object now is to determine which colony contains the cDNA that encodes (and expresses) the particular protein of interest.

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