By Ken Komiya, Masayuki Yamamura, John A. Rose (auth.), Ashish Goel, Friedrich C. Simmel, Petr Sosík (eds.)

This ebook constitutes the completely refereed post-conference court cases of the 14th overseas assembly on DNA Computing, DNA 14, held in Prague, Czech Republic, in June 2008.

The 15 revised complete papers provided have been rigorously reviewed and chosen from fifty nine submissions. Their themes contain theoretical versions of biomolecular computing, demonstrations of biomolecular computing techniques, self-assembly platforms, DNA nanostructures and nanomachines, biotechnological and different purposes of DNA computing, and different comparable themes.

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Chem. Asian J. jp 2 Institute for Advanced Biosciences, Keio University, 403-1 Daihoji, Tsuruoka, Yamagata, 997-0017, Japan Institute for Bioinformatics Research and Development (BIRD), Japan Science and Technology Agency (JST) Abstract. Synthetic biology is an emerging research area that aims to investigate natural biological phenomena and reconstruct complex artificial biological systems. Recent development of genetic engineering such as multiple gene assembly method accelerates the synthetic biology study.

Rst proposed and experimentally demonstrated (only for a limited number of steps) a WPCR machine [1]. It was not easy to increase the number of steps because of a phenomenon called back-annealing (also known as backhybridization), where a hairpin with a longer double stranded (ds) DNA region is preferentially formed over one with a shorter ds-DNA region. Sakamoto et al. suggested a modified protocol [9] where successive transitions were carried on at the same temperature, thus preventing back-hybridization.

The five biosynthetic genes, crtE, crtB, crtI, crtY, and crtZ, were extracted from the natural carotenoid cluster of Pantoea ananatis [2,6]. Actually, these five genes were amplified from the plasmid pCAR25 which was originally constructed by Misawa [6]. 2 Random Self-assembly for Carotenoid Biosynthetic Gene Operon Each random ligation of the first two genes, crtE and crtB , followed by the fixed order of three genes crtI, crtY, and crtZ for five carotenoid biosynthetic genes was assembled as one transcriptional unit (plasmid) in a polycistronic manner by OGAB method.

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