By Daryl S. Henderson
Top drosophilists describe in step by step element all of the crucial innovations for learning Drosophila chromosomes and recommend new avenues for medical exploration. The chapters emphasize specimen education (from dissection to mounting) and canopy either polytene and mitotic/meiotic chromosomes intensive. each one totally validated and without difficulty reproducible protocol bargains a historical past creation, apparatus and reagent lists, and tips about troubleshooting and heading off pitfalls. A state-of-the-art FISH and immunolocalization method should be very important for locating how DNA series affects higher-order chromosome structure and eventually gene expression.
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Additional info for Drosophila Cytogenetics Protocols
Different chromatin loops will, of course, contain different numbers and arrangements of genes, and this will have important consequences for the distribution of (+) and (–) supercoil domains in the loop. For example, for two genes in tandem and transcribed at similar levels, supercoils generated in the intergenic region will be of similar magnitude and opposite sign and therefore mutually annihilated (see Fig. 3B, top). However, the net result on supercoiling in the chromatin loop will be similar to that depicted in Fig.
Koryakov, D. , Belyaeva, E. , Alekseyenko, A. , and Zhimulev, I. F. (1996) Alpha and beta heterochromatin in polytene chromosome 2 of Drosophila melanogaster. Chromosoma 105, 310–319. 161. , Saumweber, H. and Sedat, J. W. (1986) Spatial organization of chromosomes in the salivary gland nuclei of Drosophila melanogaster. J. Cell Biol. 102, 112–123. 162. Koltzoff, N. K. (1934) The structure of the chromosomes in the salivary glands of Drosophila. Science 80, 312. 163. Beermann, W. and Pelling, C.
Butterworth, F. , Rasch, E. , and Johnson, M. B. (1985) Is there a limit of in situ genomic replication in Drosophila? J. Exp. Zool. 234, 325–328. 172. Rasch, E. M. (1970) DNA cytophotometry of salivary gland nuclei and other tissue systems in dipteran larvae, in Introduction to Quantitative Cytochemistry (Wied, G. L. and Bahr, G. ), Academic, New York, vol. 2, pp. 357–397. 173. Lamb, M. J. (1982) The DNA content of polytene nuclei in midgut and Malpighian tubule cells of adult Drosophila melanogaster.