By Mohammad A. Jafar Mazumder, Amir Al-ahmed
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Extra resources for Materials for Biomedical Applications
DNA Transfection Transfection is the delivery of DNA, Ribonucleic acid (RNA) and macromolecules into the patient target cells. A technique originally developed to allow the infected cells uptake of purified DNA rather than by intact viral or non-viral particles. In gene therapy, the DNA can be administered either by in vivo or ex vivo. In vivo process, the gene or vector can directly be administered into the patient or target organ, and potentially can be applied to any cell. The ex vivo administration includes harvesting and cultivation of cells from patients, with in vitro gene transfer and reintroduction of transfected cells.
Moreover, the non-viral vectors can easily protect DNA from degradation in the lysosome and blood stream. The majority of non-viral vectors involve synthetic materials that offer the advantage of easy and controlled synthesis, and can easily tuned the properties of the material such as block structure, hydrophobicity, charge density, cross-linking content, and molecular weight. Additionally, synthetic polymers are available with functional groups, which can be easily modified with surface ligands to achieve targeted gene delivery via receptor- mediated endocytosis.
Belinsky, MRP subfamily transporters and resistance to anticancer agent, J. Bioenerg. Biomembr. 33 (2001) 493-501.  BC Cancer Agency, Cancer Drug Manual, [Online]. htm. [Accessed: 23-Dec-2013]. G. J. S. P. Lee, Use of metabolic pathway flux information in targeted cancer drug design, Drug Discov. Today Ther. Strateg. 1 (2004) 435-443.  American Cancer Society (ACS), Different types of chemotherapy drugs, [Online]. org/treatment/treatmentsandsideeffects/treatmenttypes/chemotherapy/che motherapyprinciplesanindepthdiscussionofthetechniquesanditsroleintreatment/chemotherapy-principles-types-ofchemo-drugs.