By Douglas C. Rees (Eds.)

This quantity covers 2 significant issues: Foundations and Membrane Protein constructions. Key gains * Foundations * Bioenergetic techniques * Channels and Receptors

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References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 20 21 23 24 24 25 28 29 29 36 37 41 42 43 I. INTRODUCTION Integral membrane proteins comprise roughly 30% of all proteins and are involved in many vital cellular processes such as environmental perception, cellular communication, and nutrient metabolism. Consequently, our understanding of cell biology will never be complete without a detailed understanding of membrane protein function and the intermolecular forces that act in the bilayer.

Clearly, however, the complexities of detergent mixing in the protein–detergent complex may be difficult to separate from the protein stability. Measurement of dissociation constants of TM helix peptides is perhaps the most promising approach for evaluating the strengths of specific interactions. , 2001). These systems offer the opportunity to compare the free energies of association for TM helix variants. The fact that the measurements must be made in detergents rather than in a lipid bilayer is a drawback.

VI. Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 20 21 23 24 24 25 28 29 29 36 37 41 42 43 I. INTRODUCTION Integral membrane proteins comprise roughly 30% of all proteins and are involved in many vital cellular processes such as environmental perception, cellular communication, and nutrient metabolism. Consequently, our understanding of cell biology will never be complete without a detailed understanding of membrane protein function and the intermolecular forces that act in the bilayer.

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