By Kazunari Taira, Kazunori Kataoka, Takuro Niidome
The improvement of gene medication according to the concept that of molecular remedy has opened new scientific horizons. among the suggestions in gene medication, gene layout and supply are specially major in scientific functions. This publication offers state of the art info on non-viral gene-delivery options, protecting a wide spectrum of disciplines that come with chemistry, molecular biology, phone biology, and pharmacokinetics. significant sections introduce molecules for gene supply and their homes; applied sciences of managed gene supply in vitro and in vivo; healing genes and the prestige of medical purposes; and the layout of genes according to present RNA know-how, with progressive advancements on the planet of RNAi proven to be key components in gene medication. This groundbreaking paintings is a useful source for researchers and engineers in genetic engineering, molecular drugs, biochemical engineering, and biotechnology.
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Additional resources for Non-viral gene therapy: gene design and delivery
Hum. Gene Ther 7:1111–1122 Allen TM, Hansen C, Martin F, Redemann C, Young AY (1991) Liposomes containing synthetic lipid derivatives of poly(ethylene glycol) show prolonged circulation half-lives in vivo. Biochim Biophys Acta 1066:29–36 Aragnol D, Leserman LD (1986) Immune clearance of liposomes inhibited by an anti-Fc receptor antibody in vivo. Proc. Natl Acad Set USA 83:2699–2703 Blume G, Cevc G (1990) Liposomes for sustained drug release in vivo. Biochim Biophys Acta 1029:91–97 Cummings J, Smyth JF (1993) Cytotoxic drug delivery.
1996; Templeton et al. 1997). Templeton et al. (1997) developed a unique cationic lipid structure, so-called BIV, using DOTAP, cholesterol and a novel formulation procedure. Nucleic acids are efﬁciently encapsulated between two bilamellar invaginated structures, BIVs. This procedure is different because it includes a brief, low-frequency sonication followed by manual extrusion through ﬁlters of decreasing pore size. 2-mm ﬁlters 30 K. Maruyama consist of aluminum oxide membranes that contain a large number of pores per surface area, including evenly spaced and sized pores, and pores with straight channels.
1998; Perrie and Gregoriadis 2000) have developed a dehydration-rehydration method to entrap plasmid DNA into liposomes. This is consistent with the notion that most of the DNA is incorporated within multilamellar vesicles and largely protected from nucleases by the bilayers. The potential of applying such liposome DNA carriers as oral delivery system for DNA vaccines was also assessed. Wheeler et al. (1999) established “stabilized plasmid-lipid particles” (SPLP), produced by detergent dialysis employing a POPC/DODAC/PEG-CerC20 lipid mixture.