By C. H. Collins, J. M. Grange, M. D. Yates

Show description

Read Online or Download Organization and Practice in Tuberculosis Bacteriology PDF

Best nonfiction_12 books

A Broader Mission for Liberal Education. Baccalaureate Address, Delivered in Agricultural College ... North Dakota [FACSIMILE]

Prime quality FACSIMILE replica: Worst, John H. (John Henry): A Broader challenge For Liberal schooling. Baccalaureate handle, introduced In Agricultural university . .. North Dakota : Facsimile: initially released by means of Agricultural collage, N. D in 1901? . booklet might be published in black and white, with grayscale photographs.

Perspectives on Earthquake Geotechnical Engineering: In Honour of Prof. Kenji Ishihara

This ebook deals a vast standpoint on vital themes in earthquake geotechnical engineering and provides experts and those who are concerned with examine and alertness a extra entire realizing concerning the quite a few subject matters. which includes eighteen chapters written through authors from the main seismic energetic areas of the area, comparable to united states, Japan, Canada, Chile, Italy, Greece, Portugal, Taiwan, and Turkey, the booklet displays assorted perspectives pertaining to the right way to investigate and reduce earthquake harm.

Additional info for Organization and Practice in Tuberculosis Bacteriology

Sample text

The mixture is allowed to stand at room temperature for 12-18 hours. Distilled water, 16 ml, is added and mixed with the digested sputum. The bottle is then centrifuged. The deposit is cultured. Trisodium phosphate-Zephir an method About 2 ml of sputum is added to a bottle containing 3 ml of a mixture of 23 per cent trisodium phosphate solution containing 2 ml per litre of 17 per cent Zephiran* * Winthrop Laboratories, New York. 46 Cultural methods (benzalkonium chloride). After blending for 2 minutes on a Vortex mixer the homogenate is allowed to stand at room temperature for up to 2 hours.

Slopes showing confluent growth or isolated colonies of a buff, yellow or orange colour should be removed for microscopical examination. Close inspection, preferably with a hand lens and under a good bench lamp, is necessary to reveal very small colonies or the effuse growth which is characteristic of some opportunist mycobacteria. In Kirchner medium tubercle bacilli grow as spherical colonies, 1-2 mm in diameter, at the bottom of the tube. The bottle should be shaken gently to observe them. They may be removed with a plastic Pasteur pipette for microscopic examination and subculture on solid medium.

The slides are then washed again with running water and the counterstain poured on. This is left for 1-2 minutes and washed off. The slides are then placed on edge to drain and allowed to dry. Alternatively each slide may be blotted dry with a fresh piece of filter paper or bacteriological blotting paper (which should not be used again) and dried either by passing them once through a bunsen flame or on a drying rack. The Tan Thiam Hok (TTH) method This is a cold staining method, developed for field use when heating slides is difficult.

Download PDF sample

Rated 4.50 of 5 – based on 21 votes